Plasmid_Backbone

Part:BBa_J73998:Design

Designed by: Junling Huo   Group: Miller Lab   (2009-12-09)


plasmid vector for Rhodobacter sphaeroides


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 4614
    Illegal suffix found at 4636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4614
    Illegal SpeI site found at 4637
    Illegal PstI site found at 4651
    Illegal NotI site found at 2468
    Illegal NotI site found at 4620
    Illegal NotI site found at 4644
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4614
    Illegal XhoI site found at 28
    Illegal XhoI site found at 3463
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4614
    Illegal suffix found at 4637
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4614
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4629
    Illegal SpeI site found at 4637
    Illegal PstI site found at 4651
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 513


Design Notes

To make it more flexible and easier to control, lacZ promoter and reporter have been removed.


Source

This vector backbone is based on pMG170/pMG171. lac promoter and reporter have been removed from the original vector.

References